cbb3 oxidase has 4 subunits NOPQ,
total residues 1129,
total protein mass 124 kDa
P is related to (or equal to?) N-fixing gene Fix-P
Sequences here are for Rb capsulatus enzyme.
Subunit N is homologous to cyt aa3 oxidase subunit 1 (22% I)
(Cytochrome aa3 cytochrome oxidase has 12 transmembrane
helices)
13 predicted here, one may be leader sequence:
N (532 res, 58,956 Da, PI 9.43):
10
20 30
40 50
60
MWDYVKLVALGVVAAIAAYAASQARDLPYMVNMVEVALAAVIALIWVLRTMGDPKPSKDE
YFDGVIRAGVIATTFWGIVGFLVAVVIAFQLAFPALNLEFGNGMLNFGRLRPLHTSAVIF
AFGGNALIASAFYVVQRTSAARLFGGTALGWFVFWGWQLIIVTAATSYLLGGSQGKEYAE
LNWHLDILVAVVWVAYLIAFLGTIFKRKEPHIYVANWFYLSFIVTIAMLHIVNNLAVPVS
IFGTKSVQLMAGVQDAMTQWWYGHNAVGFFLTAGFLGMMYYFVPKQAERPVYSYKLSIVH
FWALIFLYIWAGPHHLHYTALPDWASTLGMVMSVILWMPSWGGMINGLMTLSGAWDKLRT
DPVIRMMVVSIGFYGMSTFEGPMMSIKAVNSLSHYTDWTIGHVHSGALGWNGMITFGMLY
FLTPRLWGRSGLYSLKLVSWHFWLATIGIVLYASAMWVTGIMEGLMWREVDAQGFLVNAF
ADTVAAKFPMYVVRGVGGVLYLLGGLIMAYNLWATVAKQPKTANLAVAVPAE
More subunit N sequences
O - c-type cytochrome: no homology outside cbb3 oxidases.
(242 res, 27,068 Da, PI 4.73)
10
20 30
40 50
60
MSIMDKHHVLEKNATLLLIFAFLVVTIGGIVEIAPLFYLENTIEKVEGMRPYTPLELTGR -60
DIYIREGCYVCHSQMIRPMRDEVERYGHYSLAAESMYDRPFQWGSKRTGPDLARVGGRYS
-120
DAWHVEHLINPQSVVPESVMPSYGYLAKVPLDSTWIEDRVSTDAFVGVPYTSEMIASAKA -180
DFVAQADPNADSTTLLAGYGEKVNIRNFDGQPGLTEMDALVAYLQVLGTMVDFSTFQPVA -240
SR
More subunit O sequences
P - a diheme c-type cytochrome (297 res 31,899 kDa,
PI 4.63)
has homology to solved structures 1AOF (Nitrite reductase)
and 1CYJ (cyt c6)
10
20 30
40 50
60
MSKKPTTKKEVQTTGHQWDGIEELNTPLPRWWLWTFYATIIWGVAYSIAMPAWPIFSDKA
TPGLLGSSTRADVEKDIAKFAEMNKAVEEKLVATDLTAIAADPELVTYTRNAGAAVFRTW
CAQCHGAGAGGNTGFPSLLDGDWLHGGAIETIYTNVKHGIRDPLDPDTLLVANMPAHLTD
ELLEPAQIDEVVQYVLQISGQPADEVKATAGQQIFAENCASCHGEDAKGLVEMGAPNLTD
GIWLYGGDVATLTSTIQYGRGGVMPSWSWAADGAKPRLSEAQIRAVASYVHSLGGGQ
More subunit P sequences
Q (58 residues 6546 Da PI 6.0)
10
20 30
40 50
60
MDYHILREFADSWAALALLLTFIGAVIWAFRPGSSKVHDDIANIPFRHEDKPADHGRG
More subunit Q sequences